|Application ||WB, IHC-P, E|
|Other Accession||NP_037365, 115430252|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||59474 Da|
|Application Notes||Stk39 antibody can be used for detection of Stk39 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL.|
|Reconstitution & Storage||Stk39 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Stk39 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act as a mediator of stress-activated signals. Mediates the inhibiton of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation.|
|Cellular Location||Cytoplasm. Nucleus. Note=Nucleus when caspase-cleaved.|
|Tissue Location||Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis|
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Provided below are standard protocols that you may find useful for product applications.
Stk39 Antibody: The serine/threonine kinase Stk39 belongs to the STE20 family, a group of kinases that are known to interact with inflammation-related kinases (such as p38, JNK, NKCC1, PKC-theta, WNK and MLCK), and with transcription factor AP-1. The STE 20 family is involved in diverse biological phenomena, including cell differentiation, cell transformation/ proliferation, cytoskeleton rearrangement, and the regulation of ion transporters. STK39 contains an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain and is abundantly expressed in the brain. STK39 is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled co-transporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. Recent studies show that STK39 tend to be a novel candidate gene for autism and hypertension.
Johnston AM, Nacelli G, Gonzales LJ, et al. SPAK, a STE20/SPS1-related kinase that activates the p38 pathway. Oncogene2000; 19:4290-7.
Li Y, Hu J, Vita R, et al. SPAK kinase is a substrate and target of PKCtheta in T-cell receptor-induced AP-1 activation pathway. EMBO J.2004; 23:1112-22.
Gagnon KB, England R, and Delpire E. Characterization of SPAK and OSR1, regulatory kinases of the Na-K-2Cl cotransporter. Mol. Cell Biol.2006; 26:689-98.
Dan I, Watanabe NM, and Kasumi A. The Ste20 group kinases as regulators of MAP kinase cascades. Trends Cell. Biol.2001; 11:220-30.
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