|Application ||WB, IHC-P, E|
|Other Accession||AAH50719, 29792103|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||82016 Da|
|Application Notes||ZBTB1 antibody can be used for detection of ZBTB1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL.|
|Target/Specificity||ZBTB1; At least two isoforms of ZBTB1 are known to exist. This ZBTB1 antibody will not cross-react with ZBTB2.|
|Reconstitution & Storage||ZBTB1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZBTB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a transcriptional repressor (PubMed:20797634). Represses cAMP-responsive element (CRE)-mediated transcriptional activation (PubMed:21706167). In addition, has a role in translesion DNA synthesis. Requires for UV-inducible RAD18 loading, PCNA monoubiquitination, POLH recruitment to replication factories and efficient translesion DNA synthesis (PubMed:24657165). Plays a key role in the transcriptional regulation of T lymphocyte development (By similarity).|
|Cellular Location||Nucleus. Nucleus, nucleoplasm. Note=Localized in dot-like structures in the nucleus (PubMed:21706167) Colocalized with SMRT in nuclear bodies (PubMed:20797634). The sumoylated form is preferentially located in the nucleoplasm outside the nuclear bodies(PubMed:20797634)|
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Provided below are standard protocols that you may find useful for product applications.
ZBTB1 Antibody: ZBTB1 is a zinc finger protein that also contains BTB (BR-C, ttk and bab) domain. While little is known about this protein or the related protein ZBTB2, its expression is known to be down-regulated in human bronchial epithelial cells in response to the presence of Vanadium. Recently, expression of variant ZBTB1 protein has been associated with increased age-related macular degeneration.
Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA2002; 99:16899-903.
Li Z, Stonehuerner J, Devlin RB, et al. Discrimination of Vanadium from Zinc using gene profiling in human bronchial epithelial cells. Envir. Health Perspect.2005; 113:1747-54.
Zhang H, Morrison MA, Dewan A, et al. The NEI/NCBI dbGAP database: genotypes and haplotypes that may specifically predispose to risk of neovascular age-related macular degeneration. BMC Med. Genet.2008; 9:51.
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