|Application ||WB, IHC-P, IF, E|
|Other Accession||Q9BQ50, 47606206|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||30621 Da|
|Application Notes||TREX2 antibody can be used for detection of TREX2 by Western blot at 2.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||TREX2; This TREX2 antibody will not cross-react with the related protein TREX1.|
|Reconstitution & Storage||TREX2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TREX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Exonuclease with a preference for double-stranded DNA with mismatched 3' termini. May play a role in DNA repair.|
|Tissue Location||Detected in heart, breast, prostate, skeletal muscle, testis, uterus, bone marrow, colon, small intestine, stomach and thymus.|
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Provided below are standard protocols that you may find useful for product applications.
TREX2 Antibody: TREX2 is one of two major human 3' to 5' exonucleases which are required for checkpoint signaling after DNA damage. While the related protein TREX1 is required for ATR expression and mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis, less is known about TREX2. Like TREX1, TREX2 is ubiquitously expressed in all tissues examined. X-ray crystallography studies of TREX2-single strand DNA complexes revealed that TREX2 binds DNA as a dimer and may act to displace the second DNA strand, suggesting that TREX2 unzips and denatures double stranded DNA and feed the substrate DNA strand into the TREX2 active site. At least two isoforms of TREX2 are known to exist.
Mazur DJ and Perrino FW. Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3→5 exonucleases. J. Biol. Chem.1999; 274:19655-60.
Cortez D, Guntuku S, Qin J, et. al. ATR and ATRIP: partners in checkpoint signaling. Science2001; 294:1713-6
Crow YJ, Hayward BE, Parmar R, et al. Mutations in the gene encoding the 3-5DNA exonuclease TREX1 cause Aicardi-Goutieres syndrome at the AGS1 locus. Nat. Genet. 2006; 38:917-20
Mazur DJ and Perrino FW. Structure and expression of the TREX1 and TREX2 3'→5' exonuclease genes. J. Biol. Chem.2001; 276:14718-27.
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