|Application ||WB, IHC-P, IF, E|
|Other Accession||AAH07613, 14043246|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||62184 Da|
|Application Notes||CRMP1 antibody can be used for the detection of CRMP1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 5 µg/mL.|
|Target/Specificity||CRMP1; This antibody is expected to recognize only the longest isoform of CRMP-1.|
|Reconstitution & Storage||CRMP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CRMP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. May participate in cytokinesis.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Associated with centrosomes and the mitotic spindle during metaphase|
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Provided below are standard protocols that you may find useful for product applications.
CRMP1 Antibody: Collapsin-response mediator proteins (CRMPs) are highly expressed in the developing brain where they play major roles in axonal outgrowth, neurite differentiation, and apoptosis. Their continued expression in areas of high synaptic remodeling such as the cerebellum, hippocampus, and the olfactory system suggests that these proteins may also be involved in adult brain plasticity. CRMP-1 was initially identified as a dihydro-pyrimidinase expressed exclusively in brain; later studies have shown that it is involved with neurotrophin (NT) 3-induced neurite formation and outgrowth. CRMP-1 localization switches from axonal to somatodendritic when neurons reach functional maturity, suggesting that it is involved in early neuronal differentiation as well as in later processes related to the survival or death of the newly generated neurons.
Charrier E, Reibel S, Rogemond V, et al. Collapsin response mediator proteins (CRMPs): involvement in nervous system development and adult neurodegenerative disorders. Mol. Neurobiol.2003; 28:51-64.
Cameron HA and McKay RD. Adult neurogenesis produces a large pool of new granule cells in the dentate gyrus. J. Comp. Neurol.2001; 435:406-417.
Hamajima N, Matsuda K, Sakata S, et al. A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution. Gene.1996; 180:157-63.
Quach TT, Duchemin A-M, Rogemond V, et al. Involvement of collapsin response mediator proteins in the neurite extension induced by neurotrophins in dorsal root ganglion neurons. Mol. Cell. Neurosci.2004; 25:433-43.
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