LRFN1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q9P244 |
Other Accession | Q9P244, 189028858 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 82318 Da |
Application Notes | LRFN1 antibody can be used for detection of LRFN1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 57622 |
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Target/Specificity | LRFN1; This antibody is predicted to not cross-react with other members of the LRFN family. |
Reconstitution & Storage | LRFN1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | LRFN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | LRFN1 |
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Synonyms | KIAA1484, SALM2 |
Function | Promotes neurite outgrowth in hippocampal neurons. Involved in the regulation and maintenance of excitatory synapses. Induces the clustering of excitatory postsynaptic proteins, including DLG4, DLGAP1, GRIA1 and GRIN1 (By similarity). |
Cellular Location | Membrane; Single-pass type I membrane protein. Synapse. Postsynaptic density membrane. Note=Detected in excitatory, but not inhibitory, synaptic plasma membrane. |
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Provided below are standard protocols that you may find useful for product applications.
Background
LRFN1 Antibody: LRFN1 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while Lrfn2 and Lrfn5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system. At least two isoforms of LRFN1 are known to exist.
References
Morimura N, Inoue T, Katayama K, et al. Comparative analysis of structure, expression and PSD95-binding capacity of Lrfn, a novel family of neuronal transmembrane proteins. Gene2006; 380:72-83.
Kobe B and Kajava AV. The leucine-rich repeat as a protein recognition motif. Curr. Opin. Struct. Biol.2001; 11:725-32.
Aruga J and Mikoshiba K. Identification and characterization of Slitrk, a novel transmembrane protein family controlling neurite outgrowth. Mol. Cell Neurosci.2003; 24:117-29.
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