|Application ||WB, IHC-P, E|
|Other Accession||NP_057664, 51294|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||128995 Da|
|Application Notes||PCDH12 antibody can be used for detection of PCDH12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.|
|Target/Specificity||PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12.|
The immunogen is located within amino acids 30 - 80 of PCDH12.
|Reconstitution & Storage||PCDH12 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||PCDH12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions. Promotes homotypic calcium-dependent aggregation and adhesion and clusters at intercellular junctions. Unable to bind to catenins, weakly associates with the cytoskeleton (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Cell junction|
|Tissue Location||Expressed in highly vascularized tissues including the heart and placenta, but most tissues contain a low level of expression. Prominent expression in the spleen|
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Provided below are standard protocols that you may find useful for product applications.
PCDH12 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2, 289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling.
Frank M and Kemler R. Protocadherins. Curr. Opin. Cell Biol.2002; 14:557-62.
Telo P, Breviaro F, Huber P, et al. Identification of a novel cadherin (vascular endothelial cadherin-2) located at intercellular junctions in endothelial cells. J. Biol. Chem.1998; 28:17565-72.
Rampon C, Prandini MH, Bouillot S, et al. Protocadherin 12 (VE-cadherin 2) is expressed in endothelial, trophoblast, and mesangial cells. Exp. Cell Res.2005; 302:48-60.
Rampon C, Bouillot S, Climescu-Haulica A, et al. Protocadherin 12 deficiency alters morphogenesis and transcriptional profile of the placenta. Physiol. Genomics2008; 34:193-204.
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