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Alpha-tubulin Antibody

  • WB - Alpha-tubulin Antibody ASC10875
    Western blot analysis of Tubulin in rat brain tissue lysate with Tubulin antibody at (A) 0.5 and (B) 1 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13748
Other Accession NP_005992, 17921993
Reactivity Human, Mouse, Rat
Host Chicken
Clonality Polyclonal
Isotype IgY
Calculated MW 49960 Da
Application Notes Tubulin antibody can be used for detection of Tubulin by Western blot at 0.5 - 1 µg/mL.
Additional Information
Gene ID 7278
Target/Specificity TUBA3C;
Reconstitution & Storage Alpha-tubulin antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsAlpha-tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms TUBA2
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Testis specific. {ECO:0000269|Ref.5}.
Research Areas
Citations (0)

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Alpha-tubulin Antibody: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.


McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci.2001; 114:2723-33.
Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol.1988; 2:133-53.
Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem.2009; 109:683-693.

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$ 310.00
Cat# ASC10875
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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