|Application ||WB, IHC-P, E|
|Other Accession||Q9NRR5, 45476982|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||63853 Da|
|Application Notes||CIP75 antibody can be used for detection of CIP75 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Reconstitution & Storage||CIP75 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CIP75 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||C1orf6, CIP75, UBIN|
|Function||Plays a role in the regulation of proteasomal protein degradation. Depending on the case, may promote or inhibit proteasomal protein degradation.|
|Cellular Location||Nucleus. Cytoplasm. Endoplasmic reticulum. Cytoplasm, perinuclear region Note=Colocalizes with the proteasome, both in nucleus and cytoplasm. May associate with the endoplasmic reticulum|
|Tissue Location||Highly expressed in pancreas, kidney, skeletal muscle, heart and throughout the brain, and at lower levels in placenta, lung and liver.|
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Provided below are standard protocols that you may find useful for product applications.
CIP75 Antibody: The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.
Su V and Lau AF. Ubiquitin-like and ubiquitin-associated domain proteins: significance in proteasomal degradation. Cell. Mol. Life Sci.2009; 66:2819-33.
Davidson JD, Riley B, Burright EN, et al. Identification and characterization of an ataxin-1-interacting protein: A1Up, a ubiquitin-like protein. Hum. Mol. Genet.2000; 9:2305-12.
Li X, Su V, Kurata WE, et al. A novel Connexin43-interacting protein, CIP75, which belongs to the UbL-UBA protein family, regulates the turnover of Connexin4 J. Biol. Chem.2008; 283:5748-59.
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