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RUSC1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BVN2 |
| Other Accession | NP_001098673, 157412245 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 96444 Da |
| Application Notes | RUSC1 antibody can be used for detection of RUSC1 by Western blot at 1 µg/mL. |
| Gene ID | 23623 |
|---|---|
| Target/Specificity | RUSC1; |
| Reconstitution & Storage | RUSC1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | RUSC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RUSC1 {ECO:0000303|PubMed:30262884, ECO:0000312|HGNC:HGNC:17153} |
|---|---|
| Function | Associates with the adapter-like complex 4 (AP-4) and may therefore play a role in vesicular trafficking of proteins at the trans-Golgi network (PubMed:30262884). Signaling adapter which plays a role in neuronal differentiation (PubMed:15024033). Involved in regulation of NGF-dependent neurite outgrowth (PubMed:15024033). May play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins (By similarity). Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK (PubMed:15024033). Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway (PubMed:19365808). |
| Cellular Location | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q8BG26}. Cytoplasmic vesicle {ECO:0000250|UniProtKB:Q8BG26}. Early endosome {ECO:0000250|UniProtKB:Q8BG26}. Postsynaptic density {ECO:0000250|UniProtKB:Q8BG26}. Golgi apparatus {ECO:0000250|UniProtKB:Q8BG26}. Note=Translocated to the nuclear envelope upon stimulation with NGF (PubMed:15024033). Associated with membranes and microtubules (By similarity) {ECO:0000250|UniProtKB:Q8BG26, ECO:0000269|PubMed:15024033} |
| Tissue Location | Predominantly expressed in brain. |
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Provided below are standard protocols that you may find useful for product applications.
Background
RUSC1 Antibody: RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IκB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-κB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-κB activation pathway.
References
Matsuda S, Miyazaki K, Ichigotani Y, et al. Molecular cloning and characterization of a novel human gene (NESCA) which encodes a putative adapter protein containing SH3. Biochim. Biophys. Acta2000; 1491:321-6.
Katoh M and Katoh M. Characterization of RUSC1 and RUSC2 genes in silico. Oncol. Rep.2004; 12:933-8.
MacDonald JI, Kubu CJ, and Meakin SO. Nesca, a novel adapter, translocates to the nuclear envelope and regulates neurotrophin-induced neurite outgrowth. J. Cell Biol.2004; 164:851-62.
Napolitano G, Mirra S, Monfregola J, et al. NESCA: a new NEMO/IKKgamma and TRAF6 interacting protein. J. Cell. Phys.2009; 220:410-7.
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