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VARP Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, E |
|---|---|
| Primary Accession | Q96NW4 |
| Other Accession | NP_115515, 116063534 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 116984 Da |
| Application Notes | VARP antibody can be used for detection of VARP by Immunohistochemistry at 5 µg/mL. |
| Gene ID | 84079 |
|---|---|
| Target/Specificity | ANKRD27; |
| Reconstitution & Storage | VARP antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | VARP Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ANKRD27 |
|---|---|
| Function | May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (PubMed:16525121, PubMed:18477474). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (PubMed:23104059). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (By similarity). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (By similarity). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 (PubMed:22705394). Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (PubMed:19745841). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (PubMed:24856514). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity). |
| Cellular Location | Early endosome. Late endosome. Cytoplasmic vesicle membrane. Lysosome Cell membrane. Melanosome {ECO:0000250|UniProtKB:Q3UMR0}. Note=Colocalizes with VAMP7 in transport vesicles in the shaft of hippocampal neurons (By similarity) {ECO:0000250|UniProtKB:Q3UMR0} |
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Provided below are standard protocols that you may find useful for product applications.
Background
VARP Antibody: The VPS9 ankyrin repeat protein (VARP) binds to the Rab21, a guanine nucleotide exchange factor that plays an essential role in endocytic trafficking. VARP localizes to early endosomes and is thought to regulate endosome dynamics. VARP also interacts with TI-VAMP/VAMP7, a vesicular SNARE that mediates an exocytic pathway that is crucial to neurite growth. Depletion of VARP by RNA interference impairs neurite growth, suggesting that VARP is a positive regulator of neurite growth.
References
Zhang X, He X, Fu XY, et al. Varp is a Rab21 guanine nucleotide exchange factor and regulates endosome dynamics. J. Cell Sci. 2006; 119:1053-62
Burgo A, Sotirakis E, Simmler MC, et al. Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner in neurite growth. EMBO Rep. 2009; 10:1117-24.
Alberts P, et al. Cross talk between tetanus neurotoxin-insensitive vesicle-associated membrane protein-mediated transport and L1-mediated adhesion. Mol. Biol. Cell 2003; 14:4207-20
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