|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_056371, 7662126|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||200029 Da|
|Application Notes||SIPA1L1 antibody can be used for detection of SIPA1L1 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||SIPA1L1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SIPA1L1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Stimulates the GTPase activity of RAP2A. Promotes reorganization of the actin cytoskeleton and recruits DLG4 to F- actin. Contributes to the regulation of dendritic spine morphogenesis (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse, synaptosome. Note=Associated with the actin cytoskeleton Detected at synapses and dendritic spines of cultured hippocampal neurons (By similarity).|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
SIPA1L1 Antibody: Signal-induced proliferation associated-like protein 1 (SIPA1L1) is a member of the SIPA1 family of RapGAPs. SIPA1L1 was initially identified as a binding partner and degradation target of the E6 oncoprotein of high-risk papillomaviruses. Recently, it was discovered that Casein kinase I epsilon (CKIe), a Wnt-regulated kinase that regulates Wnt/b-catenin signaling, also can bind to the carboxy-terminus of SIPA1L1. CKIe phosphorylates SIPA1L1, thereby reducing its stability and alleviating its inhibition of Rap1, a protein required for Wnt8/CKIe-mediated gastrulation during embryogenesis, suggesting SIPA1L1 plays important roles in embryo development as well as control of cell proliferation.
Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci.2009; 100:17-23.
Gao Q, Srinivasan S, Boyer SN, et al. The E6 oncoproteins of high-risk papillomaviruses bind to a novel putative GAP protein, E6TP1, and target it for degradation. Mol. Cell Biol.1999; 19:733-44.
Tsai I-C, Amack JD, Gao Z-H, et al. A Wnt-CKI e-Rap1 pathway regulates gastrulation by modulating SIPA1L1, a Rap GTPase activating protein. Dev. Cell2007; 12:335-47.
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