|Application ||WB, IHC-P, E|
|Other Accession||NP_149113, 90652863|
|Calculated MW||43003 Da|
|Application Notes||CLPH antibody can be used for detection of CLPH by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Reconstitution & Storage||CLPH antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CLPH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Cytoplasm. Mitochondrion. Cell projection, cilium, flagellum Note=Detected in mitochondria of step 17 to 18 spermatids Associated with the mitochondrial inner membrane in step 18 spermatids (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
CLPH Antibody: CLPH (Casein-Like phosphoprotein) is a recently discovered, evolutionarily conserved, testis-specific cytoplasmic protein expressed exclusively in postmeiotic germ cells in rat spermatogenesis. CLPH is a novel casein kinase 2-phosphorylated disordered and calcium-binding protein that displays strong variation in relative expression levels during spermatogenesis. It is frequently associated with the mitochondrial inner membrane during the last steps of spermatid differentiation and is involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis.
Calvel P, Kervarrec C, Lavigne R, et al. CLPH, a novel casein kinase 2-phosphorylated disordered protein, is specifically associated with postmeiotic germ cells in rat spermatogenesis. J. Proteome Res.2009; 8:2953-65.
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