|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_862823, 32469495|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||43671 Da|
|Application Notes||TRESK antibody can be used for detection of TRESK by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||TRESK antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TRESK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation (By similarity).|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Expressed specifically in dorsal root ganglion and trigeminal ganglion neurons. Detected at low levels in spinal cord.|
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Provided below are standard protocols that you may find useful for product applications.
TRESK Antibody: TRESK (TWIK-related spinal cord K+ channel) is a two-pore domain K+ channel that induces outward rectification and functions as a background K+ channel that is abundantly expressed in the spinal cord, thymus, spleen, and leukemic T-lymphocytes. TRESK is activated by increased cytoplasmic calcium concentration through calcineurin as well as by acetylcholine and histamine. It has been suggested that TRESK is linked to acute and chronic pain by activation of calcineurin. Other studies indicate that TRESK may play a role in the regulation of T-cell function and could be an excellent target to treat T-cell originated immune dysfunction.
Sano Y, Inamura K, Miyake A, et al. A novel two-pore K+ channel, TRESK, is localized in the spinal cord. J. Biol. Chem.2003; 278:27406-12.
Han J and Kang D. TRESK channel as a potential target to treat T-cell mediated immune dysfunction. Biochem. Biophys. Res. Comm.2009; 390:1102-5.
Czirjak G, Toth ZE, and Enyedi P. The two-pore domain K+ channel, TRESK, is activated by the cytoplasmic calcium signal through calcineurin. J. Biol. Chem.2004; 279:18550-8.
Huang DY, Yu BW, and Fan QW. Roles of TRESK, a novel two-pore domain K+ channel, in pain pathway and general anesthesia. Neurosci. Bull.2008; 24:166-72.
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