|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001002295, 50541959|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 49 kDa |
Observed: 49 kDa
|Application Notes||GATA3 antibody can be used for detection of GATA3 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||GATA3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||GATA3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.|
|Tissue Location||T-cells and endothelial cells.|
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Provided below are standard protocols that you may find useful for product applications.
GATA3 Antibody: GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.
Ko LJ, Yamamoto M, Leonard MW, et al. Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Mol. Cell. Biol. 1991; 11:2778-84.
Ma GT, Roth ME, Groskopf JC, et al. GATA-2 and GATA-3 regulate trophoblast-specific gene expression in vivo. Dev. 1997; 124:907-14.
Home P, Ray S, Dutta D, et al. GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. J. Biol. Chem. 2009; 284:28729-37.
Strumpf D, Mao CA, Yamanaka Y, et al. CDX2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Dev. 2005; 132:2093-102.
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