|Application ||WB, IF, E|
|Other Accession||NP_079478, 20149496|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||26928 Da|
|Application Notes||EFHD1 antibody can be used for detection of EFHD1 by Western blot at 2 - 4 µg/mL. Antibody can also be used for immunoflourescence starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||EFHD1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||EFHD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
EFHD1 Antibody: EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.
Tominaga M and Tomooka Y. Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53-/- mouse. Biochem. Biophys. Res. Comm.2002; 297:473-9.
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