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PIAS1 Antibody

     
  • WB - PIAS1 Antibody ASC11124
    Western blot analysis of PIAS1 in human kidney tissue lysate with PIAS1 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
  • IHC - PIAS1 Antibody ASC11124
    Immunohistochemistry of PIAS1 in rat kidney tissue with PIAS1 antibody at 5 µg/mL.
  • IF - PIAS1 Antibody ASC11124
    Immunofluorescence of PIAS1 in rat kidney tissue with PIAS1 antibody at 20 µg/mL.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession O75925
Other Accession NP_057250, 7706637
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 71836 Da
Application Notes PIAS1 antibody can be used for detection of PIAS1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 8554
Target/Specificity PIAS1;
Reconstitution & Storage PIAS1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsPIAS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIAS1
Synonyms DDXBP1
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.
Cellular Location Nucleus speckle. Nucleus, PML body Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton
Tissue Location Expressed in numerous tissues with highest level in testis.
Citations (0)

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Background

PIAS1 Antibody: The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells.

References

Liu B, Liao J, Rao X, et al. Inhibition of Stat1-mediated gene activation by PIAS1. Proc. Natl. Acad. Sci. USA1998; 95: 10626-31.
Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol.2005; 5:593-605.
Kang HC, Chae JH, Jeon J, et al. PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific {alpha}-globin expression. Nuc. Acids Res.2010 Apr 26.

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$ 310.00
Cat# ASC11124
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