|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_056446, 221136774|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||66157 Da|
|Application Notes||TCTN3 antibody can be used for detection of TCTN3 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 4 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||TCTN3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TCTN3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (By similarity). May be involved in apoptosis regulation. Necessary for signal transduction through the sonic hedgehog (Shh) signaling pathway.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
TCTN3 Antibody: TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.
Reiter JF and Skarnes WC. Tectonic, a novel regulator of the Hedgehog pathwy required for both activation and inhibition. Genes Dev.2006; 20:22-7.
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