|Application ||WB, IF, ICC, E|
|Other Accession||NP_003293, 91208423|
|Calculated MW||50288 Da|
|Application Notes||TRIP6 antibody can be used for detection of TRIP6 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||TRIP6 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TRIP6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Nucleus. Cytoplasm. Note=Shuttles between nucleus and cytoplasm|
|Tissue Location||Abundantly expressed in kidney, liver and lung. Lower levels in heart, placenta and pancreas. Expressed in colonic epithelial cells. Up-regulated in colonic tumors|
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Provided below are standard protocols that you may find useful for product applications.
TRIP6 Antibody: TRIP6 (Thyroid receptor interacting protein 6) is a zyxin-related focal adhesion molecule involved in cell motility and antiapoptotic responses. TRIP6 is predominantly expressed in kidney, liver and lung. It has three LIM domains, a PDZ-binding motif, a Crk SH2-binding motif and/or other protein interacting domains. TRIP6 regulates NF-κB activation and interacts with RICK and TRAF2, proteins that are involved in TNF signaling. TRIP6 antagonizes Fas-induced apoptosis and promotes Fas-mediated cell migration in apoptosis-resistant glioma cells via Src-dependent phosphorylation of TRIP6 at Tyr-55.
Murthy KK, Clark K, Fortin Y, et al. ZRP-1, a zyxin-related protein, interacts with the second PDZ domain of the cytosolic protein tyrosine phosphatase hPTP1E. J. Biol. Chem.1999; 274:20679-87.
Kassel O, Schneider S, Heilbock C, et al. A nuclear isoform of the focal adhesion LIM-domain protein Trip6 integrates activating and repressing signals at AP-1- and NF-kappaB regulated promoters. Genes Dev.2004; 18:2518-2528.
Lai YJ, Chen CS, Lin WJ, et al. c-Src-mediated phosphorylation of TRIP6 regulates its function in lysophosphatidic acid-induced cell migration. Mol. Cell. Biol.2005; 25:5859-5868.
Li L, Bin LH, Li F, et al. TRIP6 is a RIP2-associated common signaling component of multiple NF-kB activation pathways. J. Cell. Sci.2005; 118:555-563.
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