EFCAB4B Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q9BSW2 |
Other Accession | NP_001138430, 84766 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 83193 Da |
Application Notes | EFCAB4B antibody can be used for detection of EFCAB4B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
Gene ID | 84766 |
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Target/Specificity | EFCAB4B antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human EFCAB4B. The immunogen is located within amino acids 640 - 690 of EFCAB4B. |
Reconstitution & Storage | EFCAB4B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | EFCAB4B Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CRACR2A |
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Synonyms | EFCAB4B, RAB46 {ECO:0000303|PubMed:31092 |
Function | [Isoform 1]: Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex. |
Cellular Location | [Isoform 1]: Cytoplasm |
Tissue Location | [Isoform 1]: Expressed in the Jurkat T-cell line. |
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Provided below are standard protocols that you may find useful for product applications.
Background
EFCAB4B Antibody: EFCAB4B, also known as Calcium release-activated calcium channel regulator 2A, is a novel Ca2+-binding EF-hand protein that is thought to play a key role in store-operated Ca2+ entry in T-cells by regulating CRAC channel activation. EFCAB4B acts as a cytoplasmic calcium-sensor that forms a complex with ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+ concentration. A closely related protein, EFCAB4A, is likely to play a similar role as EFCAB4B, but the detailed function of EFCAB4A is still under investigation.
References
Srikanth S, Jung HJ, Kim KD, et al. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell. Biol.2010; 12:436-46.
Srikanth S, Jung HJ, Ribalet B, et al. The intracellular loop of Orai1 plays a central role in fast inactivation of Ca2+ release-activated Ca2+ channels. J. Biol. Chem.2010; 285:5066-75.
Maruyama K, Mikawa T, and Ebashi S. Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. J. Biochem.1984; 95:511-9.
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