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TMEM59 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q9BXS4 |
| Other Accession | NP_004863, 20070191 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 36223 Da |
| Application Notes | TMEM59 antibody can be used for detection of TMEM59 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 9528 |
|---|---|
| Target/Specificity | TMEM59; |
| Reconstitution & Storage | TMEM59 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | TMEM59 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TMEM59 |
|---|---|
| Synonyms | C1orf8 |
| Function | Acts as a regulator of autophagy in response to S.aureus infection by promoting activation of LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). Acts by interacting with ATG16L1, leading to promote a functional complex between LC3 and ATG16L1 and promoting LC3 lipidation and subsequent activation of autophagy (PubMed:27273576, PubMed:23376921). Modulates the O-glycosylation and complex N- glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP (PubMed:20427278). Inhibits APP transport to the cell surface and further shedding (PubMed:20427278). |
| Cellular Location | Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Note=Mainly localizes to late endosomes/lysosomes. Probably first exported to the cell surface and then actively endocytosed to transiently localize in early endosomes on its way to the late endosomal/lysosomal compartment where it becomes quickly degraded. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
TMEM59 Antibody: Processing of the amyloid precursor protein (APP) by two different proteases, called alpha- and beta-secretase, is a central regulatory event in the generation of the amyloid beta peptide (Abeta), which has a key role in Alzheimer disease (AD) pathogenesis. TMEM59, a Golgi-localized protein, modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP. It inhibits APP transport and shedding.
References
Schöbel S, Neumann S, Seed B, et al. Expression cloning screen for modifiers of amyloid precursor protein shedding. Int. J. Dev. Neurosci.2006; 24:141-8.
Schöbel S, Neumann S, Hertweck M, et al. A novel sorting nexin modulates endocytic trafficking and alpha-secretase cleavage of the amyloid precursor protein. J. Biol. Chem.2008; 283:14257-68.
Ullrich S, Münch A, Neumann S, et al. The novel membrane protein TMEM59 modulates complex glycosylation, cell surface expression, and secretion of the amyloid precursor protein. J. Biol. Chem.2010; 285:20664-74.
Elson GC, de Coignac AB, Aubry JP, et al. BSMAP, a novel protein expressed specifically in the brain whose gene is localized on chromosome 19p12. Biochem. Biophys. Res. Commun.1999; 264:55-62.
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