|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001128667, 206597541|
|Calculated MW||56461 Da|
|Application Notes||ZIP5 antibody can be used for detection of ZIP5 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||ZIP5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZIP5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in polarized cells by carrying out serosal-to-mucosal zinc transport. Plays a role in eye development. Could regulate the BMP/TGF-beta (bone morphogenetic protein/transforming growth factor-beta) signaling pathway and modulates extracellular matrix (ECM) proteins of the sclera (PubMed:24891338). Seems to play a central role in controlling organismal zinc status (By similarity).|
|Cellular Location||Basolateral cell membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in liver, kidney, pancreas, small intestine, colon, spleen, fetal liver and fetal kidney|
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Provided below are standard protocols that you may find useful for product applications.
ZIP5 Antibody: The zinc transporter ZIP5, also known as SLC39A5, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP5 is closely related to ZIP4, and is found in many of the same tissues, including intestine, pancreas, liver, and kidney, but unlike ZIP4, ZIP5 localizes to the basolateral membrane in kidney cells and its activity is not downregulated by zinc repletion. Recent evidence suggests that ZIP4 and ZIP5 are reciprocally regulated, with translation of ZIP5 mRNA decreasing and ZIP5 protein being rapidly internalized and degraded in response to zinc deficiency.
Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50.
Eide DJ. The SLC39 family of metal ion transporters. Pflugers Arch.2004; 447:796-800.
Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta.2003; 1611:16-30.
Wang F, Kim BE, Petris MJ, et al. The mammalian Zip5 protein is a zinc transporter that localizes to the basolateral surface of polarized cells. J. Biol. Chem.2004; 279:51433-41.
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