|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001131022, 212276096|
|Calculated MW||89253 Da|
|Application Notes||LRRFIP1 antibody can be used for detection of LRRFIP1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||LRRFIP1; LRRFIP1 antibody is predicted to not cross-react with other LRRFIP family members. Three isoforms of LRRFIP1 are known to exist; this antibody will only recognize the two longer isoforms.|
|Reconstitution & Storage||LRRFIP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||LRRFIP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.|
|Cellular Location||Nucleus. Cytoplasm.|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
LRRFIP1 Antibody: LRRFIP1, also known as GC-binding factor 2 (GCF2), is a 738 amino acid transcriptional repressor that mainly plays a cytoskeletal role. It is primarily localized in the cytoplasm and preferentially binds to GC-rich dsDNA, but will also bind directly to dsRNA as well. The RNA binding domain encompasses a lysine-rich motif. LRRFIP1 interacts with the mammalian Flightless I (Fli-I) and is a key component in the cytoskeletal regulation of platelet function. LRRFIP1 and the related protein LRRFIP2 may modulate canonical WNT signaling and mediate the IRF3-induced production of type I interferon via a beta-catenin-dependent pathway.
Rikiyama T, Curtis J, Oikawa M, et al. GCF2: expression and molecular analysis of repression. Biochim. Biophys. Acta 2003; 1629:15-25.
Suriano AR, Sanford AN, Kim N, et al. GCF2/LRRFIP1 represses tumor necrosis factor α expression. Mol. Cell. Biol. 2005; 25:9073-81.
Wilson SA, Brown EC, Kingsman AJ, et al. TRIP: a novel double-stranded RNA binding protein which interacts with the leucine-rich repeat of Flightless I. Nucleic Acids Res. 1998; 26:3460-7.
Goodall AH, Burns P, Salles I, et al. Transcription profiling in human platelets reveals LRRFIP1 as a novel protein regulating platelet function. Blood 2010; 116:4646-56.
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