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LRRFIP1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q32MZ4 |
| Other Accession | NP_001131022, 212276096 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 89253 Da |
| Application Notes | LRRFIP1 antibody can be used for detection of LRRFIP1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 9208 |
|---|---|
| Target/Specificity | LRRFIP1; LRRFIP1 antibody is predicted to not cross-react with other LRRFIP family members. Three isoforms of LRRFIP1 are known to exist; this antibody will only recognize the two longer isoforms. |
| Reconstitution & Storage | LRRFIP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | LRRFIP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LRRFIP1 |
|---|---|
| Synonyms | GCF2, TRIP |
| Function | Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding. |
| Cellular Location | Nucleus. Cytoplasm. |
| Tissue Location | Ubiquitously expressed. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
LRRFIP1 Antibody: LRRFIP1, also known as GC-binding factor 2 (GCF2), is a 738 amino acid transcriptional repressor that mainly plays a cytoskeletal role. It is primarily localized in the cytoplasm and preferentially binds to GC-rich dsDNA, but will also bind directly to dsRNA as well. The RNA binding domain encompasses a lysine-rich motif. LRRFIP1 interacts with the mammalian Flightless I (Fli-I) and is a key component in the cytoskeletal regulation of platelet function. LRRFIP1 and the related protein LRRFIP2 may modulate canonical WNT signaling and mediate the IRF3-induced production of type I interferon via a beta-catenin-dependent pathway.
References
Rikiyama T, Curtis J, Oikawa M, et al. GCF2: expression and molecular analysis of repression. Biochim. Biophys. Acta 2003; 1629:15-25.
Suriano AR, Sanford AN, Kim N, et al. GCF2/LRRFIP1 represses tumor necrosis factor α expression. Mol. Cell. Biol. 2005; 25:9073-81.
Wilson SA, Brown EC, Kingsman AJ, et al. TRIP: a novel double-stranded RNA binding protein which interacts with the leucine-rich repeat of Flightless I. Nucleic Acids Res. 1998; 26:3460-7.
Goodall AH, Burns P, Salles I, et al. Transcription profiling in human platelets reveals LRRFIP1 as a novel protein regulating platelet function. Blood 2010; 116:4646-56.
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