|Application ||WB, IF, ICC, E|
|Other Accession||NP_65071, 31543543|
|Calculated MW||115483 Da|
|Application Notes||ZIMP10 antibody can be used for detection of ZIMP10 by Western blot at 0.5 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||ZMIZ1; At least three isoforms are known to exist; this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members.|
|Reconstitution & Storage||ZIMP10 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZIMP10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA1224, RAI17, ZIMP10|
|Function||Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation.|
|Cellular Location||Nucleus speckle. Cytoplasm|
|Tissue Location||Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.|
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Provided below are standard protocols that you may find useful for product applications.
ZIMP10 Antibody: ZIMP10, also known as ZMIZ1, is a novel PIAS (protein inhibitor of activated signal transducer and activator of transcription)-like protein initially identified as a transcriptional co-activator of the androgen receptor (AR). ZIMP10 and the related protein ZIMP7 interact with PIAS3 and enhances AR-mediated transcription. Later experiments showed that ZIMP10 is also a co-activator of the p53 tumor suppressor. Mice deficient in ZIMP10 result in embryonic lethality by E10.5; these embryos reveal severe defects in the reorganization of the yolk sac vascular plexus, indicating that ZIMP10 plays an important role in proper vascular development.
Sharma M, Li X, Wang Y, et al. hZimp10 is an androgen receptor co-activator and forms a complex with sUMO-1 at replication foci. EMBO J. 2003; 22:6101-14.
Beliakoff J and Sun Z. Zimp7 and Zimp10, two novel PIAS-like proteins, function as androgen receptor coregulators. Nucl. Recept. Signal. 2006; 4:e017.
Lee J, Beliakoff J, and Sun Z. The novel PIAS-like protein hZimp10 is a transcriptional co-activator of the p53 tumor suppressor. Nuc. Acids Res. 2007; 35:4523-34
Beliakoff J, Lee J, Ueno H, et al. The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development. Mol. Cell. Biol. 2008; 28:282-92.
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