|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_851850, 31317218|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||42753 Da|
|Application Notes||PRR5 antibody can be used for detection of PRR5 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||PRR5; PRR5 antibody is predicted to not cross-react with other Protor protein family members.|
|Reconstitution & Storage||PRR5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||PRR5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer.|
|Tissue Location||Most abundant in kidney and liver. Also highly expressed in brain, spleen, testis and placenta. Overexpressed in several colorectal tumors.|
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Provided below are standard protocols that you may find useful for product applications.
PRR5 Antibody: Proline-rich protein 5 (PRR5), also known as Protor-1, is a 388 amino acid protein in Protor family, is thought to act as a tumor suppressor in breast and colorectal tumorigenesis. PRR5 is widely expressed and possesses two RICTOR interaction sites and a C-terminal Proline rich region. It promotes Rapamycin complex 2 (mTORC2) activity. There are four isoforms of PRR5 that are produced as a result of alternative splicing events and these isoforms play an important role in the modulation of platelet-derived growth factor signaling and in the regulation of PDGFR-beta expression.
Johnstone CN, Castellvi-Bel S, Chang LM, et al. PRR5 encodes a conserved proline-rich protein predominant in kidney: analysis of genomic organization, expression, and mutation status in breast and colorectal carcinomas. Genomics 2005; 85:338-51.
Pearce LR, Huang X, Boudeau J, et al. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. Biochem. J. 2007; 405:513-22.
Woo SY, Kim DH, Jun CB, et al. PRR5, a novel component of mTOR complex 2, regulates platelet-derived growth factor receptor expression and signaling. J. Biol. Chem. 2007; 282:25604-12.
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