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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PRR5L Antibody   

PRR5L Antibody

     
  • WB - PRR5L Antibody ASC11317
    Western blot analysis of PRR5L in 3T3 cell lysate with PRR5L antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide
  • ICC - PRR5L Antibody ASC11317
    Immunocytochemistry of PPR5L in 293 cells with PPR5L antibody at 10 µg/mL.
  • IF - PRR5L Antibody ASC11317
    Immunofluorescence of PPR5L in3T3 cells with PPR5L antibody at 20 µg/mL.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, E
Primary Accession Q6MZQ0
Other Accession NP_001153639, 237757351
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 40836 Da
Application Notes PRR5L antibody can be used for detection of PRR5L by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 79899
Target/Specificity PRR5L; At least three isoforms of PRR5L are known to exist; this antibody will recognize only isoforms a and b. PRR5L antibody will not cross-react with PPR5.
Reconstitution & Storage PRR5L antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsPRR5L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRR5L
Synonyms PROTOR2
Function Associates with the mTORC2 complex that regulates cellular processes including survival and organization of the cytoskeleton (PubMed:17461779). Regulates the activity of the mTORC2 complex in a substrate-specific manner preventing for instance the specific phosphorylation of PKCs and thereby controlling cell migration (PubMed:22609986). Plays a role in the stimulation of ZFP36-mediated mRNA decay of several ZFP36- associated mRNAs, such as TNF-alpha and GM-CSF, in response to stress (PubMed:21964062). Required for ZFP36 localization to cytoplasmic stress granule (SG) and P-body (PB) in response to stress (PubMed:21964062).
Research Areas
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Background

PRR5L Antibody: Proline-rich protein 5-like (PRR5L), also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2). PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.

References

Thedieck K, Polak P, Kim ML, et al. 2007. PRAS40 and PRR5-like protein are new mTOR interactors that regulate apoptosis. PLoS One 2007; 2:e1217.
Pearce LR, Huang X, Boudeau J, et al. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. Biochem. J. 2007; 405:513-22.

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$ 320.00
Cat# ASC11317
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