|Application ||WB, IHC-P, IF, E|
|Other Accession||EAW90125, 44662813|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||17251 Da|
|Application Notes||TMEM88 antibody can be used for detection of TMEM88 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||TMEM88; At least two isoforms of TMEM88 are known to exist; this antibody will recognize both isoforms.|
|Reconstitution & Storage||TMEM88 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TMEM88 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits the Wnt/beta-catenin signaling pathway. Crucial for heart development and acts downstream of GATA factors in the pre-cardiac mesoderm to specify lineage commitment of cardiomyocyte development.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
TMEM88 Antibody: Transmembrane protein 88 (TMEM88) is a two-transmembrane-type protein whose C-terminal tail has been shown to bind the PDZ domain of Dishevelled (Dvl), one of the key components in Wnt signaling pathways. TMEM88 attentuated the Wnt/beta-catenin signaling induced by Wnt-1 ligand in a dose-dependent manner and knockdown of TMEM88 by RNAi increased Wnt activity, suggesting that TMEM88 plays a role in regulating Wnt signaling in a context-dependent manner.
Lee HJ, Finklestein D, Li X, et al. Identification of transmembrane protein 88 (TMEM88) as a dishevelled-binding protein. J. Biol. Chem. 2010; 285:41549-56.
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