|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_653245, 31543825|
|Calculated MW||34751 Da|
|Application Notes||SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SPATA4; At least two isoforms of SPATA4 are known to exist; this antibody will detect both isoforms. SPATA4 antibody is predicted to not cross-react with SPATA protein family members|
|Reconstitution & Storage||SPATA4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SPATA4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
SPATA4 Antibody: The spermatogenesis associated protein 4 (SPATA4) protein, also known as TSARG1, is highly expressed in testis, and to a much lower extent in other tissues. SPATA4 contains a highly conserved DUF1042 domain, but no significant homology with any other known protein. SPATA4 is thought to play an important role in the development of cryptorchid testis and is a testis-specific apoptosis candidate oncogene.
Liu SF, Lu GX, Liu G, et al. Cloning of a full-length cDNA of human testis-specific spermatogenic cell apoptosis inhibitor TSARG2 as a candidate oncogene. Biochem. Biophys. Res. Commun. 2004; 319:32-40
Liu SF, Ai C, Ge ZQ, et al. Molecular cloning and bioinformatic analysis of SPATA4 gene. J. Biochem. Mol. Biol. 2005; 38:739-47.
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