|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_653245, 31543825|
|Application Notes||SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SPATA4; At least two isoforms of SPATA4 are known to exist; this antibody will detect both isoforms. SPATA4 antibody is predicted to not cross-react with SPATA protein family members|
|Reconstitution & Storage||SPATA4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SPATA4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Widely expressed. EMBL; AY040204; AAK72463.1; -; mRNA EMBL; BC021731; AAH21731.1; -; mRNA CCDS; CCDS3826.1; - RefSeq; NP_653245.2; NM_144644.3 UniGene; Hs.481235; - ProteinModelPortal; Q8NEY3; - SMR; Q8NEY3; - BioGrid; 126337; 3 IntAct; Q8NEY3; 2 MINT; MINT-8201599; - STRING; 9606.ENSP00000280191; - iPTMnet; Q8NEY3; - PhosphoSitePlus; Q8NEY3; - BioMuta; SPATA4; - DMDM; 48474486; - PaxDb; Q8NEY3; - PeptideAtlas; Q8NEY3; - PRIDE; Q8NEY3; - DNASU; 132851; - Ensembl; ENST00000280191; ENSP00000280191; ENSG00000150628 GeneID; 132851; - KEGG; hsa:132851; - UCSC; uc003iuo.2; human CTD; 132851; - DisGeNET; 132851; - GeneCards; SPATA4; - HGNC; HGNC:17333; SPATA4 HPA; HPA019220; - MIM; 609879; gene neXtProt; NX_Q8NEY3; - OpenTargets; ENSG00000150628; - PharmGKB; PA38232; - eggNOG; ENOG410IJ0V; Eukaryota eggNOG; ENOG410ZE7H; LUCA GeneTree; ENSGT00510000048442; - HOGENOM; HOG000007428; - HOVERGEN; HBG054406; - InParanoid; Q8NEY3; - OMA; FPRNINR; - OrthoDB; EOG091G0I7I; - PhylomeDB; Q8NEY3; - TreeFam; TF323506; - BioCyc; ZFISH:ENSG00000150628-MONOMER; - GenomeRNAi; 132851; - PRO; PR:Q8NEY3; - Proteomes; UP000005640; Chromosome 4 Bgee; ENSG00000150628; - CleanEx; HS_SPATA4; - ExpressionAtlas; Q8NEY3; baseline and differential Genevisible; Q8NEY3; HS GO; GO:0005737; C:cytoplasm; IEA:Ensembl InterPro; IPR010441; CH_2 PANTHER; PTHR12509; PTHR12509; 1 Pfam; PF06294; CH_2; 1 1: Evidence at protein level; Complete proteome; Polymorphism; Reference proteome CHAIN 1 305 Spermatogenesis-associated protein 4 /FTId=PRO_0000072101 VARIANT 149 149 Y -> C (in dbSNP:rs17062589) /FTId=VAR_051369 CONFLICT 70 70 N -> D (in Ref. 2; AAH21731) SEQUENCE 305 AA; 34751 MW; 7A3D1DCDC3FAC976 CRC64; MAAAGQEKGY LTQTAAALDK SPSLSPQLAA PIRGRPKKCL VYPHAPKSSR LSRSVLRWLQ GLDLSFFPRN INRDFSNGFL IAEIFCIYYP WELELSSFEN GTSLKVKLDN WAQLEKFLAR KKFKLPKELI HGTIHCKAGV PEILIEEVYT LLTHREIKSI QDDFVNFTDY SYQMRLPLVS RSTVSKSIKD NIRLSELLSN PNMLTNELKA EFLILLHMLQ RKLGRKLNPE WFDVKPTVGE VTLNHLPAQA SGRRYNLKVK RGRVVPVLPN IGSGGSSHRE IHVKQAGQHS YYSAMKPIRN MDKKP|
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Provided below are standard protocols that you may find useful for product applications.
SPATA4 Antibody: The spermatogenesis associated protein 4 (SPATA4) protein, also known as TSARG1, is highly expressed in testis, and to a much lower extent in other tissues. SPATA4 contains a highly conserved DUF1042 domain, but no significant homology with any other known protein. SPATA4 is thought to play an important role in the development of cryptorchid testis and is a testis-specific apoptosis candidate oncogene.
Liu SF, Lu GX, Liu G, et al. Cloning of a full-length cDNA of human testis-specific spermatogenic cell apoptosis inhibitor TSARG2 as a candidate oncogene. Biochem. Biophys. Res. Commun. 2004; 319:32-40
Liu SF, Ai C, Ge ZQ, et al. Molecular cloning and bioinformatic analysis of SPATA4 gene. J. Biochem. Mol. Biol. 2005; 38:739-47.
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