|Application ||WB, E|
|Other Accession||NP_660306, 21687119|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||61109 Da|
|Application Notes||SPATA18 antibody can be used for detection of SPATA18 by Western blot at 1 µg/mL.|
|Target/Specificity||SPATA18; At least three isoforms of SPATA18 are known to exist; this antibody will detect the two longest isoforms. SPATA18 antibody is predicted to not cross-react with other SPATA family members.|
|Reconstitution & Storage||SPATA18 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SPATA18 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix.|
|Cellular Location||Cytoplasm. Mitochondrion outer membrane Note=Localizes to the cytoplasm under normal conditions (PubMed:21264228). Relocalizes to mitochondrion outer membrane following cellular stress. Colocalizes with BNIP3 and BNIP3L at the mitochondrion outer membrane (PubMed:22292033)|
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Provided below are standard protocols that you may find useful for product applications.
SPATA18 Antibody: SPATA18, also known as SPETEX-1, was initially identified as a cytoplasmic protein of elongate spermatids that is thought to be involved in differentiation process of elongate spermatids to mature spermatozoa. Recent experiments have shown that SPATA18 is a transcriptional target of p53 and p63. It has also been suggested to play a role in mitochondria quality control by inducing intramitochondrial lysosome-like organella that eliminate oxidized mitochondrial proteins.
Iida H, Ichinose J, Kaneko T, et al. Complementary DNA cloning of rat spetex-1, a spermatid-expressing gene-1, encoding a 63 kDa cytoplasmic protein of elongate spermatids. Mol. Reprod. Dev. 2004; 68:385-93.
Bornstein C, Brosh R, Molchadsky A, et al. SPATA18, a spermatogenesis-associated gene, is a novel transcriptional target of p53 and p63. Mol. Cell Biol. 2011; 31:1679-89
Miyamoto Y, Kitamura N, Nakamura Y, et al. Possible existence of lysosome-like organella within mitochondria and its role in mitochondrial quality control. PLoS One 2011; 6:e16054.
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