|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055621, 55741719|
|Calculated MW||161225 Da|
|Application Notes||RUSC2 antibody can be used for detection of RUSC2 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||RUSC2; At least three isoforms are known to exist; this antibody will detect all three isoforms. This antibody is predicted to not cross-react with RUSC1.|
|Reconstitution & Storage||RUSC2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||RUSC2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Cytoplasm, cytosol. Note=Cytosolic punctate distribution. Also observed in the perinuclear region|
|Tissue Location||Widely expressed, with highest levels in brain and testis.|
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Provided below are standard protocols that you may find useful for product applications.
RUSC2 Antibody: RUSC2, also known as Iporin, shares with the related protein RUSC1 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC2 is a rab1-interacting protein that also interacts with GM130, another rab1-interacting protein. RUSC2 interacts with specific rab1 isoforms with different rab-binding specificity. It has been suggested that RUSC2 may function as a link between the targeting of ER derived vesicles triggered by the rab1 GTPase and a signaling pathway composed of proteins containing SH3 and/or poly-proline regions.
Bayer M, Fischer J, Kremerskthen J, et al. Identification and characterization of Iporin as a novel interaction partner for rab1. BMC Cell Biol. 2005; 29:6:15.
Katoh M and Katoh M. Characterization of RUSC1 and RUSC2 genes in silico. Oncol. Rep. 2004; 12:933-8
Fukuda M, Kobayashi H, Ishibashi K, et al. Genome-wide investigation of the rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 2011; 36:155-70
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