|Application ||WB, ICC, E|
|Other Accession||Q5BN46, 115430217|
|Calculated MW||15260 Da|
|Application Notes||PIERCE1 antibody can be used for detection of PIERCE1 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL.|
|Target/Specificity||C9orf116; PIERCE1 antibody is predicted to not cross-react with other UPF0691 family members.|
|Reconstitution & Storage||PIERCE1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||PIERCE1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
PIERCE1 Antibody: PIERCE1, also known as C9orf116, is an important p53 target gene contributing to normal DNA damage response and may play crucial roles in maintaining genomic integrity against genotoxic stresses, including UVC irradiation. PIERCE1 exists as two alternatively spliced isoforms and is predominantly expressed in human small cell lung cancer. PIERCE1 knockdown induces down-regulation of proapoptotic genes including Ei24, Apaf1 and PTEN, suggesting that PIERCE1 is a proapoptotic gene. It is helpful for in-depth understanding of p53 pathways and the design of new cancer therapies.
Sung YH, Kim HJ, Devkota S, et al. Pierce1, a novel p53 target gene contributing to the ultraviolet-induced DNA damage response. Cancer Res. 2010; 70:10454-63.
Zhou BB and Elledge SJ. The DNA damage response: putting checkpoints in perspective. Nature 2000; 408:433-9.
Sung YH, Kim HJ, Lee HW, et al. Identification of a novel Rb-regulated gene associated with the cell cycle. Mol. Cells 2007; 24:409-15.
Kakiuchi S, Daigo Y, Tsunoda T, et al. 2003. Genome-wide analysis of organ-preferential metastasis of human small cell lung cancer in mice. Mol. Cancer Res. 1:485-99.
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