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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NCLN Antibody   

NCLN Antibody

  • WB - NCLN Antibody ASC11465
    Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 µg/mL in (A) the absence and (B) the presence of blocking peptide.
  • IHC - NCLN Antibody ASC11465
    Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 µg/mL.
  • IF - NCLN Antibody ASC11465
    Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q969V3
Other Accession NP_064555, 51873031
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 62974 Da
Application Notes NCLN antibody can be used for detection of NCLN by Western blot at 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.
Additional Information
Gene ID 56926
Target/Specificity NCLN;
Reconstitution & Storage NCLN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsNCLN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart
Research Areas
Citations (0)

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NCLN Antibody: Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.


Haffner C, Frauli M, Topp S, et al. Nicalin and its binding partner Nomo are novel Nodal signaling antagonists. EMBO J. 2004; 23:3041-50.
Dettmer U, Kuhn PH, Abou-Ajram C, et al. Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex. J. Biol. Chem. 2010; 285:26174-81.

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$ 310.00
Cat# ASC11465
(40 western blots)
Availability: 5-7days
Bulk Size
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