|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_064555, 51873031|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||62974 Da|
|Application Notes||NCLN antibody can be used for detection of NCLN by Western blot at 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.|
|Reconstitution & Storage||NCLN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||NCLN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass membrane protein|
|Tissue Location||Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart|
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Provided below are standard protocols that you may find useful for product applications.
NCLN Antibody: Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.
Haffner C, Frauli M, Topp S, et al. Nicalin and its binding partner Nomo are novel Nodal signaling antagonists. EMBO J. 2004; 23:3041-50.
Dettmer U, Kuhn PH, Abou-Ajram C, et al. Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex. J. Biol. Chem. 2010; 285:26174-81.
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