|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_009093, 24307933|
|Calculated MW||52865 Da|
|Application Notes||TFEB antibody can be used for detection of TFEB by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.|
|Reconstitution & Storage||TFEB antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TFEB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.|
|Cellular Location||Cytoplasm. Nucleus Note=Mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). In macrophages, translocates into the nucleus upon live S.enterica infection (PubMed:27184844).|
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Provided below are standard protocols that you may find useful for product applications.
TFEB Antibody: The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors. Recent studies have shown that lysosomal biogenesis is regulated by TFEB, which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1. Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes.
Carr CS and Sharp PA. A helix-loop-helix protein related to the immunoglobulin E box-binding proteins. Mol. Cell Biol. 1990; 10:4384-8.
Davis IJ, Hsi BL, Arroyo JD, et al. Cloning of an alpha-TFEB fusion in renal tumors harboring the t(6;11)(p21;q13) chromosome translocation. Proc. Natl. Acad. Sci. USA 2003; 100:6051-6.
Sardiello M, Palmieri M, di Ronza A, et al. A gene network regulating lysosomal biogenesis and function. Science 2009; 325:473-7.
Pena-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC, et al. Regulation of TFEB and V-ATPases my mTORC1. EMBO J. 2011; 30:3242-58
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