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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ABCA7 Antibody   

ABCA7 Antibody

     
  • WB - ABCA7 Antibody ASC11494
    Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 µg/mL.
  • ICC - ABCA7 Antibody ASC11494
    Immunocytochemistry of ABCA7 in 293 cells with ABCA7 antibody at 5 µg/mL.
  • IF - ABCA7 Antibody ASC11494
    Immunofluorescence of ABCA7 in 293 cells with ABCA7 antibody at 20 µg/mL.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, E
Primary Accession Q8IZY2
Other Accession NP_061985, 150417984
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 236 kDa

Observed: 240 kDa
Application Notes ABCA7 antibody can be used for detection of ABCA7 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 5 µg/mL.
Additional Information
Gene ID 10347
Target/Specificity ABCA7; ABCA7 antibody is predicted to not cross-react with other ABC1 subfamily proteins.
Reconstitution & Storage ABCA7 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsABCA7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCA7
Function Plays a role in phagocytosis by macrophages of apoptotic cells. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocytes differentiation.
Cellular Location Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q91V24}; Multi-pass membrane protein. Early endosome membrane {ECO:0000250|UniProtKB:Q91V24}; Multi-pass membrane protein. Note=Localizes to cell membrane ruffles and phagocytic cups of macrophages stimulated with C1q or apoptotic cells. Localizes to the cytoplasm of resting macrophages, probably in Golgi and endosomes. Localizes to the apical brush border of cells in the proximal tubules of kidney (By similarity). Isoform 2 may localize to the endoplasmic reticulum (PubMed:14592415) {ECO:0000250|UniProtKB:Q91V24, ECO:0000269|PubMed:14592415}
Tissue Location Expressed in leukocytes (at protein level). Widely expressed. Highly expressed in myelo-lymphatic tissues including peripheral leukocytes, thymus, spleen and bone marrow Isoform 2 is more abundant in lymph node, spleen, thymus and trachea than isoform 1 which is more strongly expressed in brain and bone marrow.
Research Areas
Citations (0)

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Background

ABCA7 Antibody: ATP-binding cassette (ABC) transporters are an evolutionarily conserved family that use ATP hydrolysis to catalyze the transport of various molecules across cell membranes. They are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCA7, a member of the ABC1 subfamily, is a full-size ABC transporter consisting of two sets of the multiple membrane-spanning domains plus the Walker motifs for the ATP interaction. ABCA7 shows the highest homology to ABCA1, an essential molecule for cholesterol homeostasis. The high expression levels of ABCA7 in peripheral leukocytes, thymus, spleen and bone marrow suggests a role in the immune system lipid homeostasis.

References

Kaminski WE, Orsó E, Diederich W, et al. Identification of a novel human sterol-sensitive ATP-binding cassette transporter (ABCA7). Biochem. Biophys. Res. Commun. 2000; 273:532-8.
Abe-Dohmae S, Ueda K, and Yokoyama S. ABCA7, a molecule with unknown function. FEBS Lett. 2006; 580:1178-82
Ikeda Y, Abe-Dohmae S, Munehira Y, et al. 2003. Post-transcriptional regulation of human ABCA7 and its function for the apoA-I-dependent lipid release. Biochem. Biophys. Res.Commun. 311:313-8.
Iwamoto N, Abe-Dohmae S, Sato R, et al. ABCA7 expression is regulated by cellular cholesterol through the SREBP2 pathway and associated with phagocytosis. J. Lipid Res. 2006; 47:1915-27.

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$ 320.00
Cat# ASC11494
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