|Application ||WB, IF, ICC, E|
|Other Accession||NP_443070, 431822382|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||41971 Da|
|Application Notes||SEPT1 antibody can be used for detection of SEPT1 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 5 µg/mL.|
|Target/Specificity||SEPT1; At least two isoforms of SEPT1 are known to exist. This antibody is predicted to not cross-react with other Septin protein family members.|
|Reconstitution & Storage||SEPT1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SEPT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody. Note=Remains at the centrosomes and the nearby microtubules throughout mitosis Localizes to the midbody during cytokinesis|
|Tissue Location||Expressed at high levels in lymphoid and hematopoietic tissues.|
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Provided below are standard protocols that you may find useful for product applications.
SEPT1 Antibody: SEPT1 (also known as Septin 1), is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. SEPT1 was initially identified through yeast two-hybrid screening as an interaction partner for the serine/threonine kinase Aurora-B. During mitosis, SEPT1 localizes to the spindle pole, suggesting that it may play a role in cytokinesis and chromosome congression.
Qi M, Yu W, Liu S, et al. Septin1, a new interaction partner for human serine/threonine kinase aurora-B. Biochem. Biophys. Res. Commun. 2005; 336:994-1000.
Russell SE and Hall PA. Septin genomics: a road less travelled. Biol. Chem. 2011; 392:763-7.
Zhu J, Qi ST, Wang YP, et al. Septin1 is required for spindle assembly and chromosome congression in mouse oocytes. Dev. Dyn. 2011; 240:2281-9.
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