|Application ||WB, IF, E|
|Other Accession||NP_005566, 61742777|
|Calculated MW||113 kDa|
|Application Notes||LNPEP antibody can be used for detection of LNPEP by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||LNPEP; At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.|
|Reconstitution & Storage||LNPEP antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||LNPEP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.|
|Cellular Location||Cell membrane; Single-pass type II membrane protein. Note=In brain only the membrane- bound form is found. The protein resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Localization may be determined by dileucine internalization motifs, and/or by interaction with tankyrases|
|Tissue Location||Highly expressed in placenta, heart, kidney and small intestine. Detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon|
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Provided below are standard protocols that you may find useful for product applications.
LNPEP Antibody: LNPEP, also known as insulin-responsive aminopeptidase (IRAP), was initially identified as an abundant protein that is associated with specialized endosomes termed GLUT4 storage vesicles (GSVs) in adipocytes. The luminal domain of LNPEP contains an aminopeptidase activity that is involved in peptide hormone processing and antigenic peptide processing. LNPEP is also a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to the GSVs.
Keller SR, Scott HM, Mastick CC, et al. Cloning and characterization of a novel insulin-regulated membrane aminopeptidase from Glut4 vesicles. J. Biol. Chem. 1995; 270:23612-8.
Wallis MG, Lankford MF, and Keller SR. Vasopressin is a physiological substrate for the insulin-regulated aminopeptidase IRAP. Am. J. Physiol. Endocrinol. Metab. 2007; 293:E1092-102.
Saveanu L, Carroll O, Weimershaus M, et al. IRAP identifies an endosomal compartment required for MHC class I cross-presentation. Science 2009; 325:213-7.
Jordens I, Molle D, Xiong W, et al. Insulin-regulated aminopeptidase is a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to specialized insulin-regulated vesicles. Mol. Biol. Cell 2010; 21:2034-44.
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