|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_644805, 21618340|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 85 kDa|
|Application Notes||STAT3 antibody can be used for detection of STAT3 by Western blot at 1 - 2 µg/mL.|
|Reconstitution & Storage||STAT3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||STAT3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.|
|Cellular Location||Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3 Identified in a complex with LYN and PAG1|
|Tissue Location||Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
STAT3 Antibody: STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b, and STAT6), STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT3 signals are pivotal to the proliferation and differentiation of neural stem cells and also participate in neuronal regeneration and cancers of the nervous system.
Schaefer TS, Sanders LK, and Nathans D. Cooperative transcriptional activity of Jun and Stat3b, a short form of Stat3. Proc. Natl. Acad. Sci. USA 1995; 92:9097-101.
Leonard WJ and O'Shea JJ. Jaks and STATs: biological implications. Annu. Rev. Immunol. 1998; 16:293-322.
Schindler C and Darnell JE Jr. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu. Rev. Biochem. 1995; 64:621-51.
Rajan P. STATus and context within the mammalian nervous system. Mol. Med. 2011; 17:965-73
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