|Application ||WB, E|
|Other Accession||NP_036382, 21361416|
|Calculated MW||22 kDa|
|Application Notes||RRAS2 antibody can be used for detection of RRAS2 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||RRAS2; Two isoforms of RRAS2 exists as a result of alternative splicing event.|
|Reconstitution & Storage||RRAS2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||RRAS2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion.|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS)|
|Tissue Location||Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle Moderate levels in lung and liver; low levels in brain, kidney, and pancreas.|
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Provided below are standard protocols that you may find useful for product applications.
RRAS2 Antibody: Activating mutations and overexpression of classical Ras subfamily members (K-RAS, N-RAS and H-RAS) have been widely investigated as key events in the development of human cancers. The RRAS subfamily of Ras-related proteins includes RRAS1, RRAS2 (TC21) and RRAS3 (M-Ras) show overall amino acid identity with the classical Ras subfamily (H-Ras, K-Ras and N-Ras) of 55–60%. RRAS2 is a small GTPbinding protein of the Ras superfamily of GTPases. It might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins. RRAS2 has high oncogenic potential and overexpression/mutations have been reported in several tumor tissues and cell lines.
Ehrhardt A, Ehrhardt GR, Guo X, et al. Ras and relatives--job sharing and networking keep an old family together. Exp. Hematol. 2002; 30:1089-106.
Lowe DG, Capon DJ, Delwart E, et al. Structure of the human and murine R-Ras genes, novel genes closely related to Ras proto-oncogenes. Cell 1987; 48:137-46
Drivas GT, Shih A, Coutavas E, et al. Characterization of four novel Ras-like genes expressed in a human teratocarcinoma cell line. Mol. Cell. Biol. 1990;10:1793-8.
Luo H, Hao X, Ge C, et al. TC21 promotes cell motility and metastasis by regulating the expression of E-cadherin and N-cadherin in hepatocellular carcinoma. Int. J. Oncol. 2010; 37:853-9
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