|Application ||WB, IF, ICC, E|
|Other Accession||NP_057026, 34147676|
|Calculated MW||Predicted: 46 kDa |
Observed: 42 kDa
|Application Notes||MTERFD1 Antibody can be used for detection of MTERFD1 by Western blot starting at 1 µg/mL. Antibody can also be used for Immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||MTERFD1; At least three isoforms of MTERFD1 are known to exist. MTERFD1 antibody is predicted to not cross-react with other MTERFD protein family members.|
|Reconstitution & Storage||MTERFD1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||MTERFD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds promoter DNA and regulates initiation of transcription. Required for normal mitochondrial transcription, and for normal assembly of mitochondrial respiratory complexes. Required for normal mitochondrial function (By similarity).|
|Tissue Location||Highly expressed in heart, liver, kidney and testis. Detected at lower levels in brain, spleen and lung|
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Provided below are standard protocols that you may find useful for product applications.
MTERFD1 Antibody: Members of the mTERF (mitochondrial transcription termination factor) family, are mitochondrial proteins that are believed to be transcription termination factors. MTERFD1 is composed of three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It binds promoter DNA and acts as a mitochondrial transcription regulator. It has been suggested that only the phosphorylated form of MTERFD1 has transcription termination activity. A closely related mTERF family member, MTERFD3, is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription.
Fernandez-Silva P, et al. The human mitochondrial transcription termination factor (mTERF) is a multizipper protein but binds to DNA as a monomer, with evidence pointing to intramolecular leucine zipper interactions. EMBO J. 1997; 16:1066-79.
Hyvarinen AK, Pohjoismaki JL, Holt IJ, et al. Overexpression of MTERFD1 or MTERFD3 impairs the completion of mitochondrial DNA replication. Mol. Biol. Rep. 2011; 38:1321-8.
Prieto-Martin A, Montoya J, Martinez-Azorin F, et al. Phosphorylation of rat mitochondrial transcription termination factor (mTERF) is required for transcription termination but not for binding to DNA. Nucleic Acids Res. 2004; 32:2059-68.
Park CB, Asin-Cayuela J, Camara Y, et al. MTERF3 is a negative regulator of mammalian mtDNA transcription. Cell 2007; 130:273-85.
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