|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_874369, 36287069|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 51, 54, 56, 60 kDa |
Observed: 53 kDa
|Application Notes||KAT5 antibody can be used for detection of KAT5 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||KAT5; Multiple isoforms of KAT5 are known to exist.|
|Reconstitution & Storage||KAT5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||KAT5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2. Promotes FOXP3 acetylation and positively regulates its transcriptional repressor activity (PubMed:17360565).|
|Cellular Location||Nucleus. Nucleus, nucleolus. Cytoplasm, perinuclear region. Note=Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies|
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Provided below are standard protocols that you may find useful for product applications.
KAT5 Antibody: KAT5 belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and non-histone proteins. KAT5 has been shown to play a role in DNA repair and apoptosis and is thought to play an important role in signal transduction and autophagy.
Kamine J, Elangovan B, Subramanian T, et al. Identification of a cellular protein that specifically interacts with the essential cysteine region of the HIV-1 Tat transactivator. Virology 1996; 216:357-66.
Kimura A and Horikoshi M. Tip60 acetylates six lysines of a specific class of core histones in vitro. Genes Cells 1998; 3:789-800.
Ikura T, Ogryzko VV, Grigoriev M, et al. Involvement of the TIP70 histone acetylase complex in DNA repair and apoptosis. Cell 2000; 102:463-73.
Lin SY, Li TY, Liu Q, et al. GSK3-TIP60-ILK1 signaling pathway links growth factor deprivation to autophagy. Science 2012; 336:477-81.
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