|Application ||WB, E|
|Other Accession||NP_002515, 4505451|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 21 |
Observed: 20 KDa
|Application Notes||N-RAS antibody can be used for detection of N-RAS by Western blot at 0.5 – 1 µg/mL.|
|Target/Specificity||NRAS; N-RAS antibody is predicted to not cross-react with H-RAS|
|Reconstitution & Storage||N-RAS antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||N-RAS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus|
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Provided below are standard protocols that you may find useful for product applications.
N-RAS Antibody: Activating mutations and overexpression of classical Ras subfamily members (N-RAS, H-RAS, and K-RAS) have been widely investigated as key events in the development of human cancers. The N-RAS protein shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. N-RAS, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
Ehrhardt A, Ehrhardt GR, Guo X, et al. Ras and relatives--job sharing and networking keep an old family together. Exp. Hematol. 2002; 30:1089-106.
Swarthout JT, Lobo S, Farh L, et al. DHHC9 and GCP9 constitute a human protein fatty acyltransferase with specificity for H- and N-Ras. J. Biol. Chem. 2005; 280:31141-8.
Malaney S and Daly RJ. The ras signaling pathway in mammary tumorigenesis and metastasis. J. Mammary Gland Biol. Neoplasia 2001; 6:101-13.
Rodenhuis S. ras and human tumors. Semin Cancer Biol. 1992; 3:241-7
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