|Application ||WB, IHC-P, E|
|Other Accession||NP_001135888, 215490011|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 37 kDa |
Observed: 35 kDa
|Application Notes||AIMP1 antibody can be used for detection of AIMP1 by Western blot at 0.5 - 1 µg/ml.|
|Target/Specificity||AIMP1; AIMP1 antibody is human, mouse and rat reactive. At least two isoforms of AIMP1 are known to exist; this antibody will recognize both isoforms. AIMP1 antibody is predicted to not cross-react with AIMP2.|
|Reconstitution & Storage||AIMP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||AIMP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1- mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.|
|Cellular Location||Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle, secretory vesicle. Secreted Endoplasmic reticulum. Golgi apparatus. Note=Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.|
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Provided below are standard protocols that you may find useful for product applications.
AIMP1 (Endothelial monocyte-activating polypeptide II, EMAP2) is a proinflammatory cytokine for monocytes and granulocytes (1). It is specifically induced by apoptosis and is involved in the control of angiogenesis, inflammation, and wound healing (1,2). AIMP1 was identified as one of three auxiliary factors of the mammalian aminoacyl tRNA synthetase (ARS) complex. It binds and facilitates the catalytic reaction of arginyl-tRNA synthetase (2,3). Recent studies show that CD23 plays an essential role in the AIMP1-induced immune response and might be a target in the treatment of inflammatory diseases (4).
Park SG, Jung KH, Lee JS, et al. Precursor of pro-apoptotic cytokine modulates aminoacylation activity of tRNA synthetase. J. Biol. Chem. 1999; 274:16673–6.
Park SG, Shin H, Shin YK, et al. The novel cytokine p43 stimulates dermal fibroblast proliferation and wound repair. Am. J. Pathol. 2005; 166:387–98.
Quevillon S, Agou F, Robinson JC, et al. The p43 component of the mammalian multi-synthetase complex is likely to be the precursor of the endothelial monocyte-activating polypeptide II cytokine. J. Biol. Chem. 1997; 272:32573–9.
Kwon HS, Park MC, Kim DG, et al. Identification of CD23 as a functional receptor for the proinflammatory cytokine AIMP1/p43. J. Cell Sci. 2012; 125:4620-9.
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