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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MAP1LC3A Antibody   

MAP1LC3A Antibody

     
  • WB - MAP1LC3A Antibody ASC11726
    Western blot analysis of MAP1LC3A in HeLa cell lysate with MAP1LC3A antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
  • IHC - MAP1LC3A Antibody ASC11726
    Immunohistochemistry of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 5 µg/mL.
  • IF - MAP1LC3A Antibody ASC11726
    Immunofluorescence of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 20 µg/mL.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9H492
Other Accession NP_852610, 31563518
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 14 kDa

Observed: 21 kDa
Application Notes MAP1LC3A antibody can be used for detection of MAP1LC3A by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 84557
Target/Specificity MAP1LC3A; MAP1LC3A antibody is human specific. At least two isoforms of MAP1LC3A are known to exist. MAP1LC3A antibody is predicted to not cross-react with MAP1LC3B or MAP1LC3C.
Reconstitution & Storage MAP1LC3A antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsMAP1LC3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP1LC3A
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Cellular Location Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes
Tissue Location Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
Research Areas
Citations (0)

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Background

Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3A belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3A is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3A, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3).

References

Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.
Halpain S and Dehmelt L. The MAP1 family of microtubule-associated proteins. Genome Biol. 2006; 7:224.
He H, Dang Y, Dai F, et al. Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J. Biol. Chem. 2003; 278:29278-87.

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$ 310.00
Cat# ASC11726
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(40 western blots)
Availability: 5-7days
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