|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_067638, 22547184|
|Calculated MW||Predicted: 96 kDa |
Observed: 110 kDa
|Application Notes||TRPV4 antibody can be used for detection of TRPV4 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||TRPV4; TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.|
|Reconstitution & Storage||TRPV4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||TRPV4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Cell junction, adherens junction. Note=Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum Isoform 5: Cell membrane.|
|Tissue Location||Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology: C type (canonical), V type (vanilloid receptor related), M type (melastatin related) and P type (PKD) (1). TRPV4, belongs to the V type subfamily and plays a role in systemic osmoregulation (2,3). TRPV4 is a calcium channel multi-pass membrane protein activated by various stimuli, including thermal stress, fatty acid metabolites and hypotonicity (3). TRPV4 is highly expressed in lung and kidney and widely expressed in brain. It plays an important role in regulating neural excitability (4).
Birnbaumer L, Yildirim E and Abramowitz J. A comparison of the genes coding for canonical TRP channels and their M, V and P relatives. Cell Calcium 2003; 33:419-32.
Alessandri-Haber N, Dina OA, Yeh JJ, et al. Transient receptor potential vanilloid 4 is essential in chemotherapy-induced neuropathic pain in the rat. J. Neurosci. 2004; 24:4444-52.
Liedtke W. TRPV4 plays an evolutionary conserved role in the transduction of osmotic and mechanical stimuli in live animals. J. Physiol. 2005; 567:53-8.
Shibasaki K, Suzuki M, Mizuno A, et al. Effects of body temperature on neural activity in the hippocampus: regulation of resting membrane potentials by transient receptor potential vanilloid 4. J. Neurosci. 2007; 27:1566–75.
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