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HMGB1 Antibody

  • WB - HMGB1 Antibody ASC11770
    Western blot analysis of HMGB1 in human brain tissue lysate with HMGB1 antibody at (A) 1 and (B) 2 µg/ml
  • IHC - HMGB1 Antibody ASC11770
    Immunohistochemistry of HMGB1 in human lung tissue with HMGB1 antibody at 5 µg/mL.
  • IF - HMGB1 Antibody ASC11770
    Immunofluorescence of HMGB1 in human lung tissue with HMGB1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09429
Other Accession NP_002119, 4504425
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 24 kDa

Observed: 24 kDa
Application Notes HMGB1 antibody can be used for detection of HMGB1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 3146
Target/Specificity HMGB1; HMGB1 antibody is human, mouse and rat reactive.
Reconstitution & Storage HMGB1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsHMGB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMGB1
Synonyms HMG1
Function DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity).
Cellular Location Nucleus. Chromosome.
Citations (0)

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The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2). However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS)-induced endotoxemia and sepsis (3). HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4).


Smerdon MJ and Isenberg I. Interactions between the subfractions of calf thymus H1 and nonhistone chromosomal proteins HMG1 and HMG2. Biochemistry 1976; 15:4242-7.
Stros M. HMGB proteins: interactions with DNA and chromatin. Biochim. Biophys. Acta. 2010; 1799:101-13.
Wang H, Bloom O, Zhang M, , et al. HMG-1 as a late mediator of endotoxin lethality in mice. Science 1999; 285:248-51.
van Zoelen MA, Yang H, Florquin S, et al. Role of toll-like receptors 2 and 4, and the receptor for advanced glycation end products in high-mobility group box 1-induced inflammation in vivo. Shock 2009; 31:280-4.

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$ 310.00
Cat# ASC11770
Availability: 5-7days
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