|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_000483, 90421313|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 163 kDa |
Observed: 160 kDa
|Application Notes||CFTR antibody can be used for detection of CFTR by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||CFTR; CFTR antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||CFTR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||CFTR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation.|
|Cellular Location||Early endosome membrane; Multi-pass membrane protein. Cell membrane; Multi- pass membrane protein. Note=In the oviduct and bronchus, detected on the apical side of epithelial cells, but not associated with cilia.|
|Tissue Location||Expressed in the respiratory airway, including bronchial epithelium, and in the female reproductive tract, including oviduct (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
The cystic fibrosis transmembrane conductance regulator (CFTR) protein is a member of the ATP-binding cassette (ABC) transporter superfamily, and a member of the MRP subfamily that is involved in multi-drug resistance (1,2). CFTR functions as a chloride channel and controls the regulation of other transport pathways (3). Mutations in this gene are associated with the autosomal recessive disorder cystic fibrosis, the most common, fatal, inherited disease of caucasian populations (1).
Kerem B, Rommens JM, Buchanan JA, et al. Identification of the cystic fibrosis gene: genetic analysis. Science 1989; 245:1073-80.
Harris A and Argent BE. The cystic fibrosis gene and its product CFTR. Semin. Cell Biol. 1993; 4:37-44.
Schwiebert EM, Benos DJ, Egan ME, et al. CFTR is a conductance regulator as well as a chloride channel. Physiol. Rev. 1999; 79:S145-66.
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