|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_003668, 27501446|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 22 kDa |
Observed: 22 kDa
|Application Notes||DENR antibody can be used for detection of DENR by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.|
|Target/Specificity||DENR; DENR antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||DENR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DENR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. Involved in translation initiation; promotes recruitmnet of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1.|
|Tissue Location||Highly expressed in heart and skeletal muscle and moderately expressed in the brain, placenta, liver and pancreas. Weakly expressed in the lung and kidney|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
DENR (density-regulated protein), also designated DRP or smooth muscle cell associated protein 3 (SMAP-3), is a 198 amino acid protein that may be involved in the translation of target mRNAs by scanning and recognition of the initiation codon (1). It plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1 (2,3). DENR contains a SUI1 domain and is found in a variety of tissues with highest levels present in skeletal and cardiac muscle. It is up-regulated in ovarian and breast cancer cells by ERBB2 overexpression (4).
Deyo JE, Chiao PJ, Tainsky MA. DRP, a novel protein expressed at high cell density but not during growth arrest. DNA Cell Biol. 1998; 17:437-47.
Skabkin MA, Skabkina OV, Dhote V, et al. Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling. Genes Dev. 2010; 24:1787-801.
Mazan-Mamczarz K and Gartenhaus RB. Post-transcriptional control of the MCT1-associated protein DENR/DRP by RNA-binding protein AUF1. Cancer Genomics Proteomics 2007; 4:233-9.
Oh JJ, Grosshans DR, Wong SG et al. Identification of differentially expressed genes associated with HER-2/neu overexpression in human breast cancer cells. Nucleic Acids Res. 1999; 27:4008-17.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.