|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_003668, 27501446|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 22 kDa |
Observed: 22 kDa
|Application Notes||DENR antibody can be used for detection of DENR by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.|
|Target/Specificity||DENR; DENR antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||DENR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DENR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. Involved in translation initiation; promotes recruitmnet of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1.|
|Tissue Location||Highly expressed in heart and skeletal muscle and moderately expressed in the brain, placenta, liver and pancreas. Weakly expressed in the lung and kidney|
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Provided below are standard protocols that you may find useful for product applications.
DENR (density-regulated protein), also designated DRP or smooth muscle cell associated protein 3 (SMAP-3), is a 198 amino acid protein that may be involved in the translation of target mRNAs by scanning and recognition of the initiation codon (1). It plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1 (2,3). DENR contains a SUI1 domain and is found in a variety of tissues with highest levels present in skeletal and cardiac muscle. It is up-regulated in ovarian and breast cancer cells by ERBB2 overexpression (4).
Deyo JE, Chiao PJ, Tainsky MA. DRP, a novel protein expressed at high cell density but not during growth arrest. DNA Cell Biol. 1998; 17:437-47.
Skabkin MA, Skabkina OV, Dhote V, et al. Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling. Genes Dev. 2010; 24:1787-801.
Mazan-Mamczarz K and Gartenhaus RB. Post-transcriptional control of the MCT1-associated protein DENR/DRP by RNA-binding protein AUF1. Cancer Genomics Proteomics 2007; 4:233-9.
Oh JJ, Grosshans DR, Wong SG et al. Identification of differentially expressed genes associated with HER-2/neu overexpression in human breast cancer cells. Nucleic Acids Res. 1999; 27:4008-17.
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