|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001231611, 347658964|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 48 kDa |
Observed: 55 kDa
|Application Notes||POU2F3 antibody can be used for detection of POU2F3 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.|
|Target/Specificity||POU2F3; POU2F3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist. This antibody will recognize both isoforms. POU2F3 antibody is predicted to not cross-react with other members of the POU domain class 2 family.|
|Reconstitution & Storage||POU2F3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||POU2F3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen.|
|Tissue Location||Specifically expressed in epidermis and cultured keratinocytes|
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Provided below are standard protocols that you may find useful for product applications.
POU2F3, also known as Epoc-1, is a member of a family of POU domain family of transcription factors (1). POU2F3 is expressed primarily in the epidermis and plays a critical role in keratinocyte proliferation and differentiation (1,2). It is a crucial transcription factor that is required for the development of sweet, umami, and bitter, but not sour taste receptor cells (3). POU2F3 is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer (4).
Yukawa K, Yasui T, Yamamoto A, et al. Epoc-1: a POU-domain gene expressed in murine epidermal basal cells and thymic stromal cells. Gene 1993; 133:163-9.
Cabral A, Fischer DF, Vermeij WP, et al. Distinct functional interactions of human Skn-1 isoforms with Ese-1 during keratinocyte terminal differentiation. J. Biol. Chem. 2003; 278:17792-9.
Matsumoto I, Ohmoto M, Narukawa M, et al. Skn-1a/Pou2f3 specifies taste receptor cell lineage. Nat. Neurosci. 2011; 14:685-7.
Zhang Z, Huettner PC, Nguyen L, et al. Aberrant promoter methylation and silencing of the POU2F3 gene in cervical cancer. Oncogene 2006; 25:5436-45.
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