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POU2F3 Antibody

  • WB - POU2F3 Antibody ASC11800
    Western blot analysis of POU2F3 in SK-N-SH cell lysate with POU2F3 antibody at (A) 1 and (B) 2 µg/ml.
  • IHC - POU2F3 Antibody ASC11800
    Immunohistochemistry of POU2F3 in rat brain tissue with POU2F3 antibody at 5 µg/mL.
  • IF - POU2F3 Antibody ASC11800
    Immunofluorescence of POU2F3 in rat brain tissue with POU2F3 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UKI9
Other Accession NP_001231611, 347658964
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 48 kDa

Observed: 55 kDa
Application Notes POU2F3 antibody can be used for detection of POU2F3 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.
Additional Information
Gene ID 25833
Target/Specificity POU2F3; POU2F3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist. This antibody will recognize both isoforms. POU2F3 antibody is predicted to not cross-react with other members of the POU domain class 2 family.
Reconstitution & Storage POU2F3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsPOU2F3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU2F3
Synonyms OTF11, PLA1
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen.
Cellular Location Nucleus.
Tissue Location Specifically expressed in epidermis and cultured keratinocytes
Citations (0)

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POU2F3, also known as Epoc-1, is a member of a family of POU domain family of transcription factors (1). POU2F3 is expressed primarily in the epidermis and plays a critical role in keratinocyte proliferation and differentiation (1,2). It is a crucial transcription factor that is required for the development of sweet, umami, and bitter, but not sour taste receptor cells (3). POU2F3 is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer (4).


Yukawa K, Yasui T, Yamamoto A, et al. Epoc-1: a POU-domain gene expressed in murine epidermal basal cells and thymic stromal cells. Gene 1993; 133:163-9.
Cabral A, Fischer DF, Vermeij WP, et al. Distinct functional interactions of human Skn-1 isoforms with Ese-1 during keratinocyte terminal differentiation. J. Biol. Chem. 2003; 278:17792-9.
Matsumoto I, Ohmoto M, Narukawa M, et al. Skn-1a/Pou2f3 specifies taste receptor cell lineage. Nat. Neurosci. 2011; 14:685-7.
Zhang Z, Huettner PC, Nguyen L, et al. Aberrant promoter methylation and silencing of the POU2F3 gene in cervical cancer. Oncogene 2006; 25:5436-45.

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$ 330.00
Cat# ASC11800
Availability: 5-7days
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