|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055860, 42734325|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 131 kDa |
Observed: 140 kDa
|Application Notes||WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.|
|Target/Specificity||WAPAL; WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist.|
|Reconstitution & Storage||WAPL antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||WAPL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||FOE, KIAA0261, WAPL|
|Function||Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister- chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.|
|Cellular Location||Isoform 2: Nucleus.|
|Tissue Location||Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.|
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Provided below are standard protocols that you may find useful for product applications.
WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1). It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1,2,4). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3,4). WAPL is a new regulator of the development and metastasis of cancerous tissue (4,5).
Kwiatkowski BA, Ragoczy T, Ehly J, et al. Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2. Exp. Cell Res. 2004; 300:223-33.
Kueng S, Hegemann B, Peters BH, et al. Wapl controls the dynamic association of cohesion with chromatin. Cell 2006; 127:955-67.
Gandhi R, Gillespie PJ, and Hirano T. Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Curr. Biol. 2006; 16:2406-17.
Oikawa K, Ohbayashi T, Kiyono T, et al. Expression of a novel human gene, human wings apart-like (hWAPL), is associated with cervical carcinogenesis and tumor progression. Cancer Res. 2004; 64:3545-9.
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