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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Vinculin Antibody   

Vinculin Antibody

     
  • IF - Vinculin Antibody ASC11803
    Immunofluorescence of RAIDD in HeLa cells with RAIDD antibody at 5 µg/ml.
  • IHC - Vinculin Antibody ASC11803
    Immunohistochemistry of LMX1A in mouse brain tissue with LMX1A Antibodyat 5 µg/mL.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, E
Primary Accession P18206
Other Accession NP_003364, 7414
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 120 kDa

Observed: 117 kDa
Application Notes Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml. For immunofluorescence start at 20 μg/mL.
Additional Information
Gene ID 7414
Target/Specificity Vinculin antibody was raised against a 23 amino acid peptide near the center of human Vinculin.

The immunogen is located within amino acids 820 - 870 of Vinculin.
Reconstitution & Storage Vinculin antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsVinculin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VCL
Function Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P12003}; Peripheral membrane protein {ECO:0000250|UniProtKB:P12003}; Cytoplasmic side {ECO:0000250|UniProtKB:P12003}. Cell junction, adherens junction {ECO:0000250|UniProtKB:P12003}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:P12003}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P85972}. Note=Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. {ECO:0000250|UniProtKB:P12003}
Tissue Location Metavinculin is muscle-specific.
Research Areas
Citations (0)

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Background

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).

References

Burridge K, Fath K, Kelly T, et al. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu. Rev. Cell Biol.1988; 4:487-525.
Gilmore AP, Jackson P, Waites GT, et al. Further characterization of the talin-binding site in the cytoskeletal protein vinculin. J. Cell Sci. 1992; 103:719-31.
Deakin NO, Ballestrem C, and Turner CE. Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA. PLoS One 2012; 7:e37990.
Goldmann WH, Auernheimer V, Thievessen I, et al. Vinculin, cell mechanics and tumour cell invasion. Cell Biol. Int. 2013; Feb 1.

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$ 320.00
Cat# ASC11803
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