|Application ||WB, IHC-P, E|
|Other Accession||NP_982272, 238231392|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 166, 220, 231 kDa |
Observed: 220, 230 kDa
|Application Notes||DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL.|
|Target/Specificity||DOCK8; DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist.|
|Reconstitution & Storage||DOCK8 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DOCK8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1). DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2). Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3).
Ruusala A and Aspenstrom P. Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology. FEBS Lett. 2004; 572:159-66.
Randall KL, Lambe T, Goodnow CC, et al. The essential role of DOCK8 in humoral immunity. Dis. Markers 2010; 29:141-50.
Engelhardt KR, McGhee S, Sinkler S, et al. Large deletions and point mutations involving the dedicator of cytokinesis 8 (DOCK8) in the autosomal recessive form of hyper-IgE syndrome. J. Allergy Clin. Immunol. 2009; 124:1289-302.
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