|Application ||WB, IHC-P, E|
|Other Accession||NP_982272, 238231392|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 166, 220, 231 kDa |
Observed: 220, 230 kDa
|Application Notes||DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL.|
|Target/Specificity||DOCK8; DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist.|
|Reconstitution & Storage||DOCK8 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DOCK8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP (By similarity). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed:25762780).|
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Provided below are standard protocols that you may find useful for product applications.
The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1). DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2). Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3).
Ruusala A and Aspenstrom P. Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology. FEBS Lett. 2004; 572:159-66.
Randall KL, Lambe T, Goodnow CC, et al. The essential role of DOCK8 in humoral immunity. Dis. Markers 2010; 29:141-50.
Engelhardt KR, McGhee S, Sinkler S, et al. Large deletions and point mutations involving the dedicator of cytokinesis 8 (DOCK8) in the autosomal recessive form of hyper-IgE syndrome. J. Allergy Clin. Immunol. 2009; 124:1289-302.
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